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Vol. 10, No. 1, February 2003

In This Issue

New Soybean Disease Biotechnology Center
Aims to Overcome Major Yield Losses

Although there have been significant improvements in soybean yields during recent decades, the percentage of the crop lost to diseases has remained virtually unchanged at 15 percent of total production. Major diseases, such as soybean cyst nematode (SCN) and sudden death syndrome, continue to plague the soybean industry, with annual losses totaling 12 million metric tons.

Jack Widholm, professor of plant physiology and genetic engineering, observes the growth of soybean cells in tissue culture. As part of the Soybean Disease Biotechnology Center, Widholm and other U of I scientists will help develop new biotechnology strategies to control major soybean diseases.

While new sources of resistance have been identified for at least some of the important soybean diseases, improved sources of resistance are still required to achieve meaningful progress in protecting growers from continued losses. Scientists also need to more fully understand how those resistance genes are expressed in the plant and how they can be incorporated into commercial varieties available to growers.

To meet this challenge, the University of Illinois recently launched the Soybean Disease Biotechnology Center with the mission of identifying and creating new sources of disease tolerance and resistance that will increase the profitability of the industry. The Center will be based at the U of I's National Soybean Research Laboratory (NSRL). Funding for this new effort was provided by the U.S. Department of Agriculture.

"The Center will bring the power of the new genetic sciences to bear upon SCN and other major soybean diseases that continue to rob growers of yield every year," says Steve Sonka, emeritus director of the NSRL. "It is expected that the Center will also become the first line of defense against new and emerging soybean diseases, such as soybean rust."

The primary goal is to reduce the annual losses to soybean diseases and to identify new sources of resistance from the genetic stocks available in USDA's National Soybean Germplasm Collection housed at the U of I.

"Additional efforts will focus on moving those resistance genes into elite soybean cultivars using the latest biotechnology techniques," Sonka says. "We also will be identifying how the movement of resistance genes into the new cultivars will affect other important attributes such as yield, protein, and oil content. There will be a high priority on disseminating this information directly to the soybean industry through web-based programs such as the Varietal Information Program for Soybeans (VIPS) and through a wide-range of publications and media outlets."

As part of this project, the NSRL has assembled two teams of U of I scientists to conduct research that directly meets those objectives. The team headed by Professors Lila Vodkin, Jack Widholm, and Steve Clough will apply leading-edge technologies to dissect the complex interactions of the soybean with specific pathogens and develop biotechnology strategies to control those diseases.

"This group will use the latest approaches in structural and functional genomics and genetic transformation to achieve that goal," Sonka says. "One of these innovative techniques involves so-called 'gene-shuffling' which has tremendous potential to rapidly generate new resistance genes. Other techniques at the cutting edge of science will allow them to determine if the genes are effective and to insert them into soybean plants for increased resistance."

"The Center will bring the power of the new genetic sciences to bear upon SCN and other major soybean diseases tht contiue to rob growers of yield every year."

The other scientific team headed by Professors Terry Niblack and Khris Lambert will focus on genetic analysis of SCN virulence and how that knowledge of pathogen virulence can be used to protect soybean resistance in the future.

"The ability to use biotechnology to predict SCN virulence through genetic markers will provide an invaluable tool for growers," Sonka says. "Success in this goal would provide growers for the first time with a wide array of soybean varieties with maximum resistance to SCN genotypes found in their specific fields. For both teams, special emphasis will be placed on communicating the results quickly and effectively to both industry and the growers."

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Survey Details Herbicide Resistant Weeds In Illinois Soybean Fields

Over the last 20 years, herbicide resistant weeds have emerged as a major problem in many Illinois soybeans fields. To date, nine different herbicide-resistant weed biotypes have been confirmed in the state. The herbicide resistance problem, however, has continued to spread across the state and possibly encompass other weed species.

Weed Scientist Christy Sprague and Graduate Assistant Jeff Bunting examine plant samples for signs of herbicide resistance. By surveying for early signs of resistance, scientists can better determine the exact scope of the problem in Illinois and pinpoint important areas fro future research.

With support from the Illinois Soybean Checkoff Board, researchers in the Department of Crop Sciences at the University of Illinois recently conducted a major survey of growers to better determine the exact scope of the problem and pinpoint important areas for future research.

" In our survey, 43 percent of the respondents reported that they had encountered problems from herbicide-resistant weeds," says Christy Sprague, weed scientist with U of I Extension and coordinator for the weeds project. "They identified 26 different weed species with resistance to nine different herbicide classes. The earliest of these problems dates all the way back to 1986."

The survey indicated that waterhemp, ragweed, common lambsquarter, and common cocklebur comprised nearly 80 percent of the herbicide-resistant species in the state. Reports over the last few years have confirmed resistance in those four species to ALS inhibitors, triazine herbicides or even both of those classes.

" The respondents also identified some of those weeds as being resistant to other herbicides, including the PPO inhibitors, such as Flexstar, Ultra Blazer, and Cobra," Sprague says. "Since that survey, we have indeed confirmed some waterhemp with resistance to the PPO inhibitors."

U of I weed scientists Aaron Hager and Pat Tranel are now conducting additional research to pinpoint the exact mechanisms at work in this PPO resistance.
" The survey also included reports of waterhemp not being effectively controlled by glyphosate," Sprague says. "Although some of this may be due to environmental conditions, there are some populations that do not seem to fit that explanation. Although no waterhemp population in the state has been confirmed as resistant to glyphosate, there remains some concern that this could become a problem in the future."

She notes that researchers in several other Midwest states are working with some waterhemp populations that have not been effectively controlled with glyphosate. They have determined that some of those populations show increased tolerance.

"One major advantage of the survey is that it allows researchers to more easily identify what new weed speices in the state ma be developing herbicide resistance."

The Illinois survey also identified resistance problems in several ther weed species that have not yet been confirmed as resistant in the state. These include horseweed, velvetleaf, morningglory, and woolly cupgrass.

" Most of these were reported by only a few respondents and may not truly be resistant," Sprague says. "In the case of horseweed, however, there are confirmed cases of resistance to ALS inhibitors in Ohio and glyphosate in Delaware, Tennessee, Kentucky, Ohio, Indiana, New Jersey, and Maryland. All of which raises some concern that this could be a potential problem in Illinois."

According to Sprague, one major advantage of the survey is that it allows researchers to more easily identify what new weed species in the state may be developing herbicide resistance.

"The results let us get a better handle on what may be some of the emerging weed problems," she says. "We also can get a heads-up on the potential for certain weed species to develop resistance to major herbicides, such as glyphosate. With that kind of information, we can better focus our research on the areas that will be the most productive in dealing with any problem that we may confront in the future."

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Illinois Center For Soy Foods Publishes Second Cookbook

A new cookbook that presents the many ways that textured soy protein can be used as a healthy and delicious ingredient in the average American kitchen has been published by the Illinois Center for Soy Foods at the University of Illinois. This illustrated, full-color publication entitled Textured Vegetable Protein in the American Kitchen is the second in an ongoing series of soy foods cookbooks.

"Textured soy protein can best be looked at as a typically American soy food," says Barbara Klein, editor of the book and co-director of the Center. "It is a quick-cooking food with the texture and nutritional value of meat. It can serve as a valuable addition to the kitchens of people who are looking for a quick and inexpensive source of protein and a way to cut back on fat in their diets."

Textured soy protein is generally made from whole or defatted soybeans that have been texturized and then ground into granules or chunks of varying sizes. Consumers can buy this product under a variety of names--textured soy protein, textured vegetable protein, textured soy flour, TSP (a registered trademark of PMS Foods LP), and TVP (a registered trademark of Archer Daniels Midland Company). For simplicity, the term "TVP was used in the cookbook.

"TVP provides a complete protein that includes all the essential amino acids," Klein says. "It is virtually fat free and has no cholesterol. It is also very low in sodium and high in dietary fiber. TVP also retains soy's isoflavones, which are special components in soy that contribute to its ability to prevent disease."

Klein notes that this new book fits well with the goal of the Center, which is to encourage consumers in the U.S. to eat more healthy products made from soy.

"With this book, we hope to inspire a broad range of people to use and enjoy this American soy food," Klein says. "We show step-by-step how average consumers can cook with TSP and still enjoy the same tastes and textured that they have grown up with."

"This new book fits well with the goal of the Center, which is to encourage consumers in the U.S. to eat more healthy products made from soy."

Textured Vegetable Protein in the American Kitchen contains a wide range of tasty recipes ranging from appetizers to desserts, as well as many appealing entrees. All the recipes were developed by Cheryl Sullivan, M.A., R.D., and extensively tested by the staff at the Center.

"With this new cookbook, we can show everyone just how easily this healthy ingredient can be added to the kinds of foods that all of us are used to eating," Klein says. "TSP is especially adaptable for use in many of our favorite comfort foods, such as chili, meatloaf, and calzones. We also have recipes for everything from carrot bread to cookies. And, most importantly, it all tastes good."

She points out that adding TSP to the diet also represents an easy way to gain the many health benefits from soy protein. The U.S. Food and Drug Administration has recently approved a health claim for soy foods that acknowledged the connection between consuming soy and decreasing the chance of developing cardiovascular disease.

" Extensive research has confirmed that eating 25 grams of soy protein per day, as part of a diet low in saturated fat and cholesterol, may reduce the risk of heart disease," Klein says. "This fact provides another example of how using TSP and other soy foods can add affordable, flavorful, and healthful components to the diet."

The cookbook was designed in an easy-to-use format, with a spiral binding that allows it to lie flat for efficient use in the kitchen. Nutritional information, including calorie, fat, carbohydrate, and protein counts, is provided for each recipe. It also contains helpful general information on buying, storing, and efficiently using TSP.

The book can be ordered at a price of $15 per copy by calling toll free at (800)345-6087. Additional information, sample recipes, and an on-line order form for the cookbook and the other title in the series, Tofu in the American Kitchen, are also available on the Internet at www.soyfoodsillinois.uiuc.edu.

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NSRL Assists in Field Testing Soy-Fortified Foods in Tajikistan

The National Soybean Research Laboratory has recently conducted field trials in the central Asian country of Tajikistan that have shown that soy can serve as an excellent source to fortify the protein content of bread and other wheat-based foods in that strategically important part of the world.

" The trials showed that soy provides much-needed protein in the foods that are staples in the diets of millions of people who have little opportunity to obtain protein from other sources," says Pradeep Khanna, associate director of the NSRL. "From its inception, we have been working with the World Initiative for Soy in Human Health (WISHH) to provide greater nutrition through food assistance programs throughout the world."

"The results of the field testing in Tajikistan proved positive both in terms of tase and ease of use in the types of foods that local resients would normally eat."

Primary funding for the WISHH program is provided by the United Soybean Board and the American Soybean Association. Additional funding has come from the Illinois Soybean Checkoff Board and state soybean organizations from across the country. The NSRL provides technical support for a wide range of projects sponsored by the program.

Tajikistan gained its independence from the former Soviet Union in 1991 and has since experienced a difficult period of adjustment. Two-thirds of the population is below poverty levels and nearly half of the children under five suffer from chronic malnutrition.

"Many of the people there depend on international food assistance from the World Food Programme and private voluntary organizations, such as Save the Children, CARE, and Catholic Relief Services," Khanna says. "These groups use tons of wheat flour in school lunch programs, yet protein and other nutritional deficiencies remain common. We hope to have a major impact on that problem by incorporating soy into the flour used in those feeding programs."

As part of the project, the NSRL has collaborated with the North American Miller=s Association to send two staff members to Tajikistan for the purpose of conducting tests on the potential for the use of soy in the food aid programs already underway. Research Specialist Megan Puzey from the NSRL was one of those who traveled there last fall to assist in the effort.

" Prior to their departure, our staff conducted extensive tests of these soy-fortified products under a variety of conditions, ranging from fire-fed clay ovens to commercial bakeries," Khanna says. "Archer Daniel Midlands Company has provided the flour, which is about 12 percent soy, 87 percent wheat, and one percent vitamin-mineral premix. This soy-fortified product can increase the protein content of food by as much as 40 percent."

The results of the field testing in Tajikistan proved positive both in terms of taste and ease of use in the types of foods that local residents would normally eat.

" The product proved especially well adapted for use in both breads and in noodles for soup," Puzey says. "In school trials, most children preferred the buns made with soy flour. Many of the children even saved the bread so that they could take it home to younger siblings who do not have access to a school meal program."

Additional tests of this soy-fortified flour were conducted by the World Food Programme in Afghanistan, where more then 9 million people are receiving U.S. food assistance. Further trials are also scheduled for Pakistan in the near future.

" Research trials like this verify the potential that soy has to do good in the world," Khanna says. "Through these efforts we are also gaining much wider international recognition for new uses of soy, especially in value-added products such as flour. This project represents a 'win-win' situation for both U.S. soybean producers and food aid recipients."

Children at an orphange in the central Asian country of Tajikistan enjoy soy-fortified buns developed at the NSRL as part of the school feeding program.

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NSRL Director Retires With Lasting Legacy Of Service

Effective on Dec. 31, 2002, National Soybean Research Laboratory (NSRL) Director Steve Sonka has retired from the University of Illinois. During his tenure at the U of I, he served as a professor of agricultural management and strategy in the Department of Agricultural and Consumer Economics.

Since 1996, Sonka has held the Soybean Industry Chair in Agricultural Strategy and served as director of the NSRL. Donald A. Holt, emeritus professor of crop physiology and former senior associate dean in the College of Agricultural, Consumer, and Environmental Sciences (ACES), will serve as interim director while a national search is underway to fill the position vacated by Sonka.

As director, Sonka has led an unprecedented expansion in the size and scope of soybean programs at the NSRL. He has proved especially effective in promoting interactions among soybean producers, researchers and industry leaders to increase dialogue among those groups that had previously no process by which to share ideas and strategies.
"Steve's ability to moderate and build consensus among distinct groups in the soybean chain made him the ideal choice for the Soybean Industry Chair of Agricultural Strategy at the U of I," says Lyle Roberts, executive director of the Illinois Soybean Checkoff Board. AHe is inordinately gifted at taking farmers' input, assessing their needs and linking those requirements with the University's capabilities and resources."

The soybean industry chair at the U of I was created through a contribution of $1.25 million from the Illinois soybean checkoff. It was established in part to provide leadership and planning so that soybean programs at the U of I would be integrated into an effective, efficient package to enhance soybean profitability.

As holder of the chair and director of the NSRL, Sonka initiated a system for regular visits to the center by industry representatives from the U.S. and around the globe, in order to maintain ongoing dialogue among all groups in the soybean value chain. He was also instrumental in organizing major conferences, such as Global Soy Forum '99, where nearly 1,700 participants from all over the world met in Chicago to help shape the future of the international soybean industry.

He has also placed a special focus on promoting soy as a human food and a source of valuable nutrition for people both in the U.S. and around the world.

" Steve was instrumental in establishing the Illinois Center for Soy Foods at the U of I,"says Pradeep Khanna, associate director of the NSRL. "The center is dedicated to developing innovative processing and marketing techniques, educating society on the advantages of a soy-enriched diet and communicating new health benefits of soy."

Under Sonka's leadership, the NSRL has also been working closely with soybean growers and the American Soybean Association on the World Initiative for Soy in Human Health (WISHH) program. This program was established in 2001 to promote the use of U.S. soy products in food aid programs and the commercial markets in developing countries around the world.

Bob Easter, dean of the College of ACES, notes that Sonka has made great contributions to the soybean industry while improving research processes at the University.
" Steve has established 'managed research' as a valid model for conducting problem-solving, collaborative research," Easter says. "Through this process and others, he has confirmed that university academics can productively engage with commodity organizations, like the soybean board, and private industry to affect positive change."

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A Farewell Message

It seems like only yesterday when I took over as director of the National Soybean Research Laboratory in January of 1996. During that time, we have accomplished many of the objectives that were set out in establishing the NSRL.

One example that immediately comes to mind is the way in which we regularly conduct scholarly activities throughout the soybean value chain. This work ranges in some cases from investigating the genome to communicating the disease susceptibility of differing varieties. In other projects, it encompasses everything from enhancing our understanding of the role of soybean meal in swine production to increasing knowledge of how soybean consumption can enhance well-being in developing countries.

Although there always will be a role for discovery research conducted by individual scholars and students, we also have focused our efforts to address many targeted needs. We currently have several managed research programs underway in which NSRL staff members have contributed immensely in conceptualizing and conducting the needed effort.

Communication throughout the soybean value chain is critically important and the academic community needs to be an integral component of that process. The faculty and staff at the NSRL have demonstrated their effectiveness in contributing to this process by successfully conducting Global Soy Forum '99, Soy2002, and numerous other meetings and events.

"As director, Sonka has led an unprecedented expansion in the size and scope of soybean programs at the NSRL."

In each of those examples, success was achieved through the collaborative efforts not just of researchers, but of committed individuals from throughout the value chain.

As I conclude this note, I need to express my particular appreciation to the board members and staff of the Illinois Soybean Program Operating Board, my colleagues on the faculty who have helped in developing new knowledge as a part of the NSRL efforts, and the staff of NSRL for their support during the time that I've had the privilege to serve as Director.

Because of this collaborative spirit, I'm sure that the NSRL will continue contributing to the success of soybeans in the near term under interim director Don Holt and well into the future once the next director is appointed.

Steve Sonka

From the Director's Desk

I am honored and privileged to serve as interim director of the National Soybean Research Laboratory while a search committee works to identify top candidates for the permanent director. It will be difficult indeed to find a new director who can meet the standards of productivity, contribution, and enlightened leadership set by the NSRL's previous director, Steve Sonka. Fortunately, Steve will still be around to offer advice and assistance. Hopefully, my experience as a farmer, researcher, and research administrator will enable me to make a useful contribution during my tenure.

The able, enthusiastic faculty, professionals, students, and clerical people associated with NSRL are briefing me on the many soy-related projects underway. It is a very impressive program being carried out by very impressive people here at the U of I and in cooperating institutions and organizations. One of the things I particularly like about it is the great communication and cooperation among NSRL faculty and staff, producer groups, and other interested organizations, such as C-FAR, and College of ACES administration.

There are no lack of interesting issues to tackle. For example, it has come to our attention that soybean producers would like to see more of the widely grown private soybean varieties entered in the University's variety trials. Also, producers and other stakeholders want new developments in soybean research programs moved more rapidly to practical use. They also want the new technology to provide Illinois and U.S. producers with a sustainable advantage in the highly competitive global market for soybeans and soy products.

Most producer organizations are increasingly interested in the way universities manage new inventions and discoveries. The question of how best to protect intellectual property through patents, trade secrets, exclusive and non-exclusive licenses, etc., is complex and the answer is different in almost every specific situation. An important goal is to see that the sponsors of research capture a reasonable return on their investment.

Among important global issues is the World Initiative for Soy in Human Health (WISHH). Soy protein can make the difference between life and death and between good and poor mental and physical development for millions of people around the world, especially those suffering devastating diseases such as HIV/AIDS. The NSRL needs to play a key role in developing a system to get that protein to the impoverished people who need it most. This effort will have both short and long-term benefits not only for the needy people, but also for producers and processors of soybeans, and for the U.S. economy in general.

I look forward to working with the NSRL team and NSRL supporters and sponsors to address these and other critically important issues.

Don Holt

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The NSRL Bulletin is published three times a year by the National Soybean Research Laboratory at the University of Illinois, 170 National Soybean Research Center, 1101 W. Peabody Drive, Urbana, IL 61801; telephone (217) 244-1706; e-mail nsrl@uiuc.edu; FAX (217) 244-1707. Donald A. Holt, interim director; Robert J. Wynstra, editor; David Riecks, photographer; Lynn Hawkinson Smith, graphic designer. Unless otherwise stated, articles may be reproduced or quoted if credit is given to the NSRL Bulletin. The National Soybean Research Laboratory at the University of Illinois is an affirmative action and equal opportunity institution.

 





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